Pacific blue fluorophore
from that of commonly used green fluorophores. Alexa Fluor 405: Excited by 405nm violet laser. Alexa Fluor 405 is an extremely dim dye, for that reason it should be only used for the brightest antigens. It is a derivated of the Cascade Blue dye. Alexa Fluor 405 conjugates show minimal spectral overlap with green fluorophores and Fluorophore type. Organic dye. Organic dye. Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye. Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye. Ex (nm) 475. 546. 496, 565. 488. Em (nm) 535. 585. 607. 710. Application. ICC, IHC. ICC, IHC. FC. FC. Conjugates available : eFluor ... Search life-sciences literature (Over 39 million articles, preprints and more)
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Comparable to Invitrogen Pacific Blue dye: Use in place of AmCyan or Horizon V500 dye: Learn more about flow antibodies for the violet laser. Blue-laser (488 nm) excitation ... PE-eFluor 610 PerCP-eFluor 710; Fluorophore type. Organic dye. Organic dye. Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye. Organic ...Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Spectra Alternative Dyes to Pacific Blue Check to add to spectra viewer Pacific Blue V450 ViaFluor 405 Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. …As compared with the aminocoumarin derivatives, the Cascade Blue fluorophore shows less spectral overlap with fluorescein , an important advantage for multicolor applications. In addition, this reactive Cascade Blue derivative has high absorptivity, is highly fluorescent and, unlike most dyes, resists quenching upon protein conjugation. Even at low degrees …Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...spectral signature of fluorophores with very similar spectra, such as Brilliant Violet 421™ and Pacific Blue™. In lieu of using PMTs, these cytometers have Avalanche Photodiode (APD) detectors, which also enables the detection of fluorophores in the near-IR and infrared wavelengths. Spillover Matrix16 авг. 2005 г. ... If calculations indicate that the protein conjugate is labeled with significantly more than seven moles of fluorophore per mole of IgG antibody, ...The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest).Blue BD Horizon V450 Pacific Blue™ BD Influx™ cell sorter Solid State 488 Green FITC Alexa Fluor® 488 Yellow PE Orange PE-Texas Red® Red PE-Cy5 PerCP-Cy5.5 Infrared PE-Cy7 Solid State 532 or 561 Yellow PE Orange PE-Texas Red® Red PE-Cy5 Infrared PE-Cy7 Solid State 640 Red APC Alexa Fluor® 647 Far Red Alexa Fluor®700 Infrared BD APC-H7 ...BD Horizon Brilliant™ Blue 700 (BB700) (Ex max 485 nm/Em max 693 nm) is a dye that was exclusively developed by BD Life Sciences as brighter alternative to PerCP-Cy5.5. This dye also has less cross-laser excitation off the 405-nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer ...Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low ...
An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant.StarBright Blue 810. 477. 802. 4. 750LP. 750LP. A488 (ex max 495, ex max 419) With similar fluorescence properties to FITC, Alexa Fluor 488 is not compatible with FITC or GFP. However, as it is brighter and more photostable, it is a great alternative, especially for intracellular staining and in fluorescence microscopy.Blue BD Horizon V450 Pacific Blue™ BD Influx™ cell sorter Solid State 488 Green FITC Alexa Fluor® 488 Yellow PE Orange PE-Texas Red® Red PE-Cy5 PerCP-Cy5.5 Infrared PE-Cy7 Solid State 532 or 561 Yellow PE Orange PE-Texas Red® Red PE-Cy5 Infrared PE-Cy7 Solid State 640 Red APC Alexa Fluor® 647 Far Red Alexa Fluor®700 Infrared BD APC-H7 ...Filipino budget carrier Cebu Pacific announced it would resume regular flights between Manila and Hanoi from next month. - VnExpress InternationalOur Fluorophore Equivalency Tool will help you find BioLegend’s equivalent fluorophores compared to other commercially available fluorophores. Simply select the name of a competitor's fluorophore from the dropdown menu to see BioLegend's equivalents. The tool also allows you to preview the excitation and emission spectra of our fluorophores ...
Brilliant violet fluorophores: ... For comparison, Pacific Blue (PB), a single molecule dye with near maximum absorption at 405 nm, has an extinction coefficient of 4.6 × 10 4 M −1 cm −1, a quantum efficiency of 78%, and a longer lifetime of 9 ns. Practically, the brightness of a fluorochrome is proportional to the extinction coefficient ...The LIVE/DEAD™ Fixable Blue Stain was selected based on its fluorescent properties to provide a bright signal when excited with a UV laser. The blue-fluorescent reactive dye has an excitation maximum of ∼350 nm, making it ideal for use with an UV laser, and an emission of ∼450 nm. By switching viability staining from a channel off of the ...…
Reader Q&A - also see RECOMMENDED ARTICLES & FAQs. When fluorochrome conjugated antibodies are . Possible cause: Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are.
Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. …We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach …
Dory’s character in “Finding Nemo” is a blue tang fish. Blue tang fish are characterized by their bright blue coloring and yellow tail. These fish are found in the Pacific and Indian Oceans.Table of Fluorochromes. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself.
BD Horizon Brilliant™ Blue 700 (BB700) (Ex max 485 Reagent Selection Guide for the Attune Flow Cytometers. The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers. brightest fluorochromes offered by BD Life Sciences.Search life-sciences literature (Over 39 milli Try the new and improved version of Full Spectrum Viewer in Cytek® Cloud! Sign Up For A Free Account. The new Full Spectrum Viewer features: More fluorescent tags to select from. Fluorescent tag signatures. Complexity™ Index. Similarity™ Index. Stain Index Reduction. Transfer fluorescent tags to experiment templates.Dory’s character in “Finding Nemo” is a blue tang fish. Blue tang fish are characterized by their bright blue coloring and yellow tail. These fish are found in the Pacific and Indian Oceans. Pacific Blue ; Pacific Green ; Pacific Orange ; Tetramethyl In vivo, is an accessory photosynthetic pigment found in blue-green algae: APC-Cy7: Allophycocyanin – Cy7: Tandem conjugate where APC is coupled to a cyanine dye (Cy7) Longest wavelength emission in the family of fluorophores excited by the red laser: A488: Alexa Fluor 488: Incredibly stable, so very popular dye: Spectrum almost identical to ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. The amine-reactive Pacific Blue™ succinimidyl ester can545 nm) fluorochrome is part of the BD family of blue (488-nm) laser dThe Alexa Fluor®-labeled antibodies can be used for mul Pacific Blue 455 V4. BV480. 480 BV510 510. eFluor506. 510. V500. 510 V8 Pacific Orange 550 V9. BV570. 570. Super Bright 600. 600 BV605 605 V11 BV650 650. Super Bright 702. 702 BV711 711 V14. BV750. 750 ... 830nm) of eac h fluorochrome providing a unique signature for each dye, this allows combinations that conventional cytometers cannot us … Reagent Clone Fluorochrome Stain Index CD4 An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant.Not for use in diagnostic or therapeutic procedures. APC-Cy7: US patent 5,714,386. Alexa Fluor® is a registered trademark, and Pacific Blue™ is a trademark of ... Reagent Selection Guide for the Attune Flow Cytometers. The Invitroge[Pacific Blue. BD Horizon V450. Alexa Fluor405. DDAPI (4′,6-diamidino-2-phenylindole) DAPI Texas Red (and Texas Red-X) Dye. Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Texas Red sulfonyl chloride is susceptible to hydrolysis and its low solubility in water may complicate ...